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KMID : 0811720020060000173
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 0 p.173 ~ p.0
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Protein Tyrosine Phosphatase Inhibitor, bpv(phen), Increases ANP Release in Beating Rabbit Atria
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Jin Jing-Yu
Kim Sung-Zoo
Kim Sun-Hee
Cho Kyung-Woo
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Abstract
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Protein kinase C activation with phorbol 12-myristate 13-acetate (PMA) increases atrial natriuretic peptide (ANP) release via isozyme specific pathway (Ruskoaho et al 1986; Cui et al 2002). The reports suggest that phosphoprotein is involved in the regulation of ANP release. The purpose of the present study was to define the effect of potassium bisperoxo (1,10-phenanthroline) oxovanadate [bpv(phen)], a protein tyrosine phosphatase inhibitor, in the regulation of atrial ANP release. Experiments have been done in beating rabbit atria allowing measurements of changes in atrial dynamics, transmural extracellular fluid translocation, cAMP efflux and ANP release. Administration of bpv(phen) increased atrial myocytic ANP release without significant changes in atrial dynamics. Atrial cyclic AMP efflux was not significantly changed. ANP increased slowly but steadily during the presence of bpv(phen). In the presence of bpv (phen), PMA increased ANP release which was not significantly different from the response without bpv (phen). Go6976, a protein kinase C selective inhibitor, blocked bpv(phen)-induced activation of ANP release. These findings suggest that bpv(phen) increases atrial myocytic ANP release via protein kinase C signaling.
Ruskoaho H, Toth M, Ganten D, Unger Th, and Lang RE. The phorbol ester induced atrial natriuretic peptide secretion is stimulated by forskolin and BAY K 8644 and inhibited by 8-bromo-cyclic GMP. Biochem Biophys Res Commun 139: 266-274, 1986.
Cui X, Wen JF, Jin JY, Xu WX, Kim SZ, Kim SH, Lee HS, and Cho KW. Protein kinase-dependent and Ca2+-independent inhibition by cAMP of atrial ANP release in beating rabbit atria. Am J Physiol 282: R1477-R1489, 2002.
This work was supported by a research grant from the KRF (2000-015-FP0023).
Source: Korean Journal of Physiology & Pharmacology.2002 Oct;6(Suppl II):S116-S116
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KEYWORD
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atrial natriuretic peptide, protein kinase C, protein tyrosine phosphatase
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